Six Lasiodiplodia species were identified as Lasiodiplodia pseudotheobromae, Lasiodiplodia theobromae, Lasiodiplodia brasiliense, Lasiodiplodia subglobosa, Lasiodiplodia citricola, and Lasiodiplodia iraniensis. 2017 Apr;121(4):452-465. doi: 10.1016/j.funbio.2016.06.004. 1). Part of the translation elongation factor (tef1) was amplified with primer pair EF1-728F and EF1-986 Carbone and Kohn42 and EF1-688F and EF1-1251R Alves et al.6. Food Research International, 82, 44-52. Para este caso específico, tal vez lo ideal sería el uso de un colector solar, como el desarrollado por Ghini (1993) en Brasil. (2005). Posteriormente, se lo reportó en India (Kannan et al., 2010), Samoa Occidental (Bourke, 1992), Bangladesh (Shamsi et al., 2010), y Filipinas (Alvindia & Gallema, 2017). First report of Lasiodiplodia pod rot disease of cacao - (Theobroma cacao L.) from Bangladesh. IOP Conference Series: Earth and Environmental Science, 752(1),012-030. Recientemente en cacao, Nurlaila et al. (2018). Punithalingam, E. Plant diseases attributed to Botryodiplodia theobromae. En brotes y pecíolos de vides, el patógeno produce picnidios que originan conidios, similar a los producidos en condiciones in vitro (Al-Saadoon et al., 2012; Figura 1C, 1E y 1F). (1980). Google Scholar. Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Science, Kunming is thanked for supporting DNA molecular experiments of this study. L. theobromae es un hongo necrótrofo asociado a graves daños en diferentes cultivos de importancia económica a nivel mundial (Salvatore et al., 2020), siendo considerado un patógeno peligroso que puede causar la muerte de plantas (Dwiastuti & Aji, 2021). Scientia agropecuaria, 249-258. Fungi were isolated into pure culture and grouped according to their culture morphology. 2000) has not always been strictly applied and species have been introduced on the basis of minor differences in only one locus. Maximum likelihood tree resulting from analysis of the combined ITS, tef1 and tub2 sequence data alignment. Wallingford, UK. The alignment and tree files were submitted to TreeBASE with reviewer’s link ( S23955). Gaps were treated as missing data. To view a copy of this license, visit Cocoa Growers' Bulletin, 12 - 21. Families, genera and species of Botryosphaeriales. Agriculture, 11(5), 396. Comparison of total length of 445 bases of ITS sequences revealed one base pair difference among L. magnoliae, L. mahajangana and L. pandanicola. Accepted number of species: Currently, 51 species names are listed for Lasiodiplodia in MycoBank and Index Fungorum (2019). Fungal Biol. Ex: Exocarpio; Me: Mesocarpio; En: Endocarpio; Pu: Pulpa; Fu: Funículo; Al: Almendras.Â, 5. F, Conidias maduras (septadas y oscuras con estrías longitudinales).Â. The authors declare no competing interests. (2006) described three new species (L. crassispora, L. venezuelensis and L. rubropurpurea) from the tropics based on ITS and tef1 sequence data and morphological characters. Actividad antifúngica del extracto etanólico de las hojas de Schinus molle sobre el crecimiento de Lasiodiplodia theobromae en condiciones de laboratório. Pest Management Science, 76(4), 1344-1352. & Phillips, A. Botryosphaeria corticola sp. Pat. Revista Árvore, 42(3), e420304. nov. Index Fungorum number: IF556217, Faces of Fungi number: FoF 05797 Fig. Denman et al. Universidad Nacional de Trujillo. Investigaciones Científicas y Agrotecnológicas para la Seguridad Alimentaria. Scale bars: c = 50 μm, d = 10 μm, e = 5 μm f, g = 10 μm. (2000) suggested that Lasiodiplodia could be a synonym of Diplodia. In: Congresso Brasileiro de Fruticultura. (2017). [ Links ], Recibido: As there is little information about L. theobromae in cocoa crops, we believe that this review will be very helpful for both technicians and researchers. AMISTAR® TOP es un funguicida que posee acción sistémica y de contacto. It is assumed that some fungi exhibit a continuum of life-styles ranging from biotrophy (or endophytic), through to necrotrophy and ultimately to saprotrophy29. The striated, pigmented, mature, ovoid conidia suggest close resemblances to Lasiodiplodia but the early development of striations in hyaline immature stage is a unique character for Barriopsis7,9. The combined dataset of ITS, tef1 and tub2 consisted of 54 taxa of Lasiodiplodia, with Diplodia mutila (CMW 7060) as the out group taxon and comprised 1267 characters including gaps after alignment. Esto provoca una desnaturalización de proteínas debido a dos factores: Las proteínas son funcionales en su estructura secundaria, terciaria o cuaternaria. Azoxystrobin. Bethesda, MD 20894, Web Policies Chiang Rai. (Eds. El gran número de hospederos conformado principalmente por cultivos de importancia agrícola, hacen de este patógeno un organismo cosmopolita y su capacidad de sobrevivir en el suelo y en restos vegetales lo vuelve difícil de controlar. De manera general, en la actualidad la denominación del género Lasiodiplodia puede ser considerado válido. Centro Nacional de Referencia Fitosanitaria.Vigilancia Epidemiológica Fitosanitaria. Plant Pathol J. Minor species of foliar fungal endophyte communities: do they matter? Biochemical changes and defence responses during the development of peach gummosis caused by Lasiodiplodia theobromae. [email protected] [ Links ], del Castillo, S. D., Parra, D., Noceda, C., & Pérez-Martínez, S. (2016). The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species, and test their pathogenicity and virulence on Persian lime plants. International Journal of Pure & Applied Bioscience, 5(6), 1024-1031. En los últimos años este fitopatógeno ha venido tomando importancia. It differs from L. iraniensis by unique fixed alleles in two loci: ITS position 463 (C); tef1 positions 554 (C), 599 (T), 681 (C), 703 (G) and differs from L. thailandica: ITS position 463 (C); tef1 positions 551 (C), 598 (C), 671 (C) 811 (C). It can be assumed that these combination of molecular markers strengthen the support for them and to separate the existing ones3,4. 93, 1–160, (2018). Histopathology of grapevine inoculated with Lasiodiplodia theobromae. Previous studies have used combined ITS and tef1 regions to clarify the taxonomy and phylogenetic relationships of species in Lasiodiplodia3,6,26 while others have used combined ITS, tef1, tub2 and rpb219. 2016 (species). However, cultures and DNA sequence data are available for only 35 species (March 2019)10,11,12. [ Links ], Borges, F. R. C., Marques, E., Macedo, M. A., Martins, I., Filho da Silva, J. G., & de Mello Marques, S. C. (2018). REBIOL, 35(2), 47-52. Soon after, Burgess et al. Tanto hongos, bacterias y levaduras han sido evaluados en el manejo de enfermedades causadas por L. theobromae en diferentes cultivos. (1980). Wir übersetzen gerade unsere neue Webseite. The internal transcribed spacer (ITS) was amplified with primer pair ITS4 and ITS540 as described in Alves et al.41. 2022 Jan 20;11(3):273. doi: 10.3390/plants11030273. Phylogenetic lineages in the Botryosphaeriales: a systematic and evolutionary framework. It is a perennial wooden oilseed crop, and also exhibits a good source of polyunsaturated fatty acids, protein and other bioactive compounds, such as tocopherols, carotenes and phytosterols (Chirinos et al. Fruit and Canopy Pathogens of Unknown Potential Risk. Etiología e histopatología de la muerte descendente de árboles de mamey [Pouteria sapota (Jacq.) 94, 1–22, (2019). Scale bars: c = 50 μm, e, f = 10 μm, g–k = 10 μm. Este producto es de uso preventivo y curativo, y actúa en sinergia con inductores de resistencia y antiestresantes. Sequences of the individual loci of ITS, tef1 and tub2 were aligned with MAFFT v. 7 online version44 using default settings. Australian Centre for International Agricultural Research (ACIAR). Throughout history, the cocoa crop (Theobroma cacao L.) has been affected by countless diseases, some of these caused by Lasiodiplodia theobromae (Pat.) The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species, and test their pathogenicity and virulence on Persian lime plants. Caracterização morfológica e patológica de Lasiodiplodia theobromae (Pat.) Phylogenetic analyses were conducted using DNA sequence data available in GenBank, but unfortunately sequences of tef1 and tub2 are not available for some species (see Table S1) and some of the sequences are shorter than expected. Plant Disease, 104(11), 3063. Sacha inchi (<i>Plukenetia volubilis</i> L.) belongs to the family Euphorbiaceae. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Phylogenetic trees from ITS and tub2 did not provided good resolution among Lasiodiplodia species. CAS  [ Links ], Netto, M. S. B., Assunção, I. P., Lima, G. S. A., Marques, M. W., Lima, W. G., et al. Glass, N. L. & Donaldson, G. C. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. En un estudio genómico y trangenómico reciente reali zado por Ali et al. First report of Lasiodiplodia theobromae causing bot gummosis in Citrus limon in Chile. Biocontrole pós-colheita da podridão de Lasiodiplodia em frutos de manga por leveduras saprofíticas. 5. [ Links ], Burgess, T. I., Barber, P. A., Mohali, S., Pegg, G., de Beer, W., & Wingfield, M. J. The remaining two isolates formed distinct lineages representing two new species. Careers. Por otro lado, la ocurrencia de pudrición en mazorcas se da principalmente por la intensa fuente de inóculo presente en el área de cultivo (Twumasi et al., 2014), debido a que el fitopatógeno aprovecha los desechos provenientes de la cosecha para sobrevivir y completar su ciclo de vida (Kuswinanti, 2019). Nooteboom, H. P. & Chalermglin, P. The Magnoliaceae of Thailand. Waste Management, 90, 72-83. Pure cultures of the fungus were prepared by single spore isolation37. & Maubl. Las conidias son hialinas en estado inmaduro, elipsoidales, unicelulares, de pared gruesa y con un contenido granular, mientras que en estado maduro son septadas con estrías longitudinales de color marrón oscuro (Hendra et al., 2019). Promputtha, I., Lumyong, S., Lumyong, P., McKenzie, E. C. & Hyde, K. D. Fungal succession on senescent leaves of Manglietia garrettii in Doi Suthep-Pui National park, northern Thailand. [ Links ], Valle-de la Paz, M., Guillén-Sánchez, D., Gijón-Hernández, A. R., Alía-Tejacal, I., López-Martínez, V., et al. FOIA Hyde (2019), Abdollahz., Zare & A.J.L. En frutos de banano, el tratamiento preventivo con T. viride 4 h antes de la inoculación con L. theobromae, reduce la pudrición en más del 60%. [ Links ], Akrofi, A. Y., Amoako-Atta, I., Acheampong, K., Assuah, M. K., & Melnick, R. L. (2016). Disclaimer, National Library of Medicine Fungicida. Lasiodiplodia Ellis & Everh., Bot. Este producto es de uso preventivo y curativo, y actúa en sinergia con inductores de resistencia y antiestresantes. Scale bars: d = 5 μm, e = 20 μm, f–j = 10 μm. Towards a natural classification of Botryosphaeriales. The maximum likelihood analysis resulted in a tree with largely the same topology as the maximum parsimony tree. Fungal Divers. Huda-Shakirah AR, Mohamed Nor NMI, Zakaria L, Leong YH, Mohd MH. [ Links ], Asman, A., Rosmana, A., Bailey, B.A., Shahin, A.S., Stream, M.D., et al. Nature, 418(6895), 289-290. Cocoa bean production worldwide 2018/19 & 2020/21, by country. Lasiodiplodia microconidia Y. Zhang ter., S. Lin, 2019 in [Wang Y et al. y M.A. 57100, © 2019 | All rights reserved. Fungal Biology, 121(4), 452-465. The Research Fund from China Postdoctoral Science Foundation (Grant No. Although three Lasiodiplodia species have been reported affecting cocoa plants, L. theobromae is the most studied species both in cocoa and other crops. CABI Publishing. Las almendras (granos) extraídas de sus frutos (mazorca), son materia prima para la industria alimenticia mundial, valorada en más de 47.000 millones de dólares en exportaciones mundiales (Vásquez et al., 2019), y es el ingrediente principal en la elaboración del chocolate, tras un proceso previo de fermentación y secado (Hartel et al., 2017). [ Links ], Alvindia, D. G., & Gallema, F. L.M. Lasiodiplodia species exhibit diverse life-styles as endophytes8,15, pathogens3,16 and saprobes3,17. B, Rama muerta (flecha roja). China, Yunnan Province, Xishuangbanna, fresh leaves of Magnolia candolii (Magnoliaceae), 26 April 2017, N. I. de Silva, S8 (Holotype - a dry culture on bamboo sticks - MFLU 19-0441), living cultures, MFLUCC 18-1121, KUMCC 17-0233. Comparison of total length of 450 bases of tef1 sequences revealed an insertion of eight bases in Lasiodiplodia magnoliae when compared to L. mahajangana and L. pandanicola (Table 1). (2008). En la Figura 4 se describen varias medidas que pueden ser implementadas en conjunto para reducir la muerte regresiva y pudrición de mazorcas en el cultivo de cacao (adaptado de Michereff et al., 2005; Tovar-Pedraza et al., 2013; Yang et al., 2021). Ball Valves; Ball Valves - Combination; Butterfly Valves; Check Valves; Gate Valves; Globe & Angle Valves; High Performance Butterfly Valves; Stop and Waste Valves Distribution – Worldwide, mostly confined to tropical and sub-tropical regions, but becoming increasingly more common in warm temperate regions. Bayesian posterior probabilities ≥0.5 (BYPP) indicated as thickened black branches. 06100, Ciudad de México. Está compuesto por . Phillips & K.D. [ Links ], Cavalcante, R. D., Lima, W.G., Martins, R. B., Tovar-Pedraza, J. M., Michereff, S. J., & Câmara, S. M. P. (2014). Fungal Diversity, 28, 1-13. Se encontró invernando en... El 14 de noviembre de 2022, el Servicio de Inspección de Sanidad Animal y Vegetal (APHIS) del USDA... La enfermedad de la nervadura amarilla es una enfermedad emergente de los cítricos que se observó... La temporada de lluvias en Venezuela incide en la sobrepoblación del caracol africano (Achatina... Dirección General de Sanidad Vegetal. Not to be confused with, J.A. Today the figure stands at 40 (Fig). Bot canker pathogens could complicate the management of Phytophthora black pod of cocoa. Isolation of endophytes was done according to the methods described by Promputtha et al.36 with modifications. Netto, M. S. et al. (2012). In recent studies, taxonomists frequently use highly variable protein coding genes such as tef1, tub2 together with ITS to construct phylogenies especially at species levels4. First leaves were washed using tap water and cut in to small pieces of leaves (5 × 5 mm2) and soaked in distilled water for 1 minute and then surfaced sterilized by dipping in 70% alcohol followed by 2% NaOCl for 30 s and finally washed with sterile distilled water for 30 s, dried and plated on Potato Dextrose Agar (PDA). [ Links ], Khanzada, M. A., Lodhi, A. M., & Shahzad, S. (2005). We were unable to observe conidia of L. endophytica (S8) in culture even after many attempts on different media. Australasian Plant Pathology, 42(5), 573-582. In Confectionery Science and Technology, Springer, Cham, 423-484. 2003). En condiciones de laboratorio, inicialmente el patógeno presenta un desarrollo micelial de color blanco, tornán dose posteriormente de color cenizo oscuro, hasta vol verse finalmente negruzco (Figura 1A y B). [ Links ], Cárdenas, N. J., Darghan, A., Sosa Rico, M. D., & Rodríguez, A. Aunque su crecimiento óptimo está entre 29 y 30 °C (Pitt, Huang, & Savocchia, 2013), el microorganismo puede esporular entre 15 y 40 °C (Shaidul et al., 2001), siendo favorecido por períodos lluviosos que estimula la masiva producción de esporas y su diseminación (Vásquez-López et al.,2009). The RAxML analysis yielded a best scoring tree with the final ML optimization likelihood value of - 4851.693940 (ln). In previous studies, phylogenetic analyses were solely based on ITS nucleotide sequences3 to identify Lasiodiplodia species. Colonies on PDA reaching 30 mm diameter after 3 days at 20–25 °C, colonies medium sparse, circular, surface slightly rough with edge entire, margin well-defined, cottony to fairly fluffy with sparse aspects, colony from above: grey to black with fluffy appearance; reverse black. Lo importante es el uso integrado de dos o más medias de manejo, pues de esta manera se tendría suceso en la reducción de enfermedades causadas por L. theobromae. The new collections suggest that Magnolia forest plants are good hosts for Lasiodiplodia species with endophytic and saprobic life-styles. En esta revisión, se ha considerado importante citar diferentes trabajos donde se ha evaluado la sensibilidad de aislados de L. theobromae obtenidos a partir de varios tejidos y especies vegetales, fungicidas usados de forma habitual en condiciones de campo (Tabla 1). (2014). (2019)] holotype of Lasiodiplodia microconidia: HMAS:255198 Hasta la presente fecha, poco se conoce al respecto de L. theobromae en el cultivo de cacao. Analysis of tef1 resulted in a better resolution of many taxa than single ITS and tub2 gene trees and showed similar phylogenetic relationships as combined ITS, tef1 and tub2 analyses. de Beer (2016), Jayasiri, E.B.G. Peach gummosis caused by Lasiodiplodia theobromae is one of the most detrimental diseases to peaches in southern China. En Ecuador hasta la fecha se ha reportado al patógeno únicamente afectando árboles de T. gileri (Evans et al., Incidencia de enfermedades fúngicas en plantaciones de cacao de las provincias orientales de Cuba.Revista de Protección Vegetal,30(2), 87-96. Gaz. Sin embargo, otras enfermedades como mal del machete (Ceratocystis cacaofunesta), así como la pudrición de la mazorca y muerte regresiva o descendente, ambas causadas por Lasiodiplodia (syn. Forest Pathology, 35(6), 385-396. Con relación a otros fungicidas y sulfatos de cobre . 21:92 (1896). [ Links ], Torres-de la Cruz, M., Guillén, G. Lasiodiplodia theobromae es uno de los principales hongos que atacan al aguacate. Amherst, Massachusetts: published by the author. This might be possible because endophytes switch their nutritional mode to saprobic when environmental conditions become unfavorable to the host or during host senescence29. Burgess, P.A. DNA was kept at 4 °C for DNA amplification and maintained at −20 °C for long term storage. El empleo de extractos vegetales o de alguno de sus derivados, pueden funcionar como fungicidas botánicos o simplemente bioestimulantes. 28, 1–13 (2008). Finalmente, la pre-inoculación en plantas de mango con dichas actinobacterias en condiciones de invernadero, pudo reducir fuertemente la severidad de la muerte regresiva causada por L. theobromae. The specimens cited in this paper are maintained at the Mae Fah Luang University Herbarium (MFLU), Chiang Rai, Thailand and Kunming Institute of Botany herbarium (HKAS), Kunming, China. (a,b) Appearance of conidiomata on twig of Magnolia species. Explorar. The family is characterized by large, ovoid to oblong, usually hyaline, aseptate ascospores and hyaline or pigmented, aseptate, one or rarely multi-septate, thick walled conidia usually with longitudinal striations4,7. Presencia y daños causados por Lasiodiplodia theobromae en los frutales de diferentes provincias y localidades de Cuba. A., Crous, C. J., De Beer, Z. W., Wingfield, M. J. Phillips (2014), I.B.L. "Combodia" redirects here. Keywords: Damm, U., Crous, P. W. & Fourie, P. H. Botryosphaeriaceae as potential pathogens of Prunus species in South Africa, with descriptions of Diplodia africana and Lasiodiplodia plurivora sp. A., et al. Fungal Divers. Morphology Characterization, Molecular Identification, and Pathogenicity of Fungal Pathogen Causing Kaffir Lime Leaf Blight in Northern Thailand. La avispilla del almendro (Eurytoma amigdali) apareció en la Región de Murcia en 2018, en Jumilla... Colombia es el primer productor mundial de uchuva, seguido por Sudáfrica. Global production of cocoa beans by region 2003/04-2020/2021. Chomnunti, P. et al. nov. Fungal Divers. in the crop of cocoa. PubMed  nov. Mycologia 99, 664–680, (2007). Aunque únicamente en condiciones in vitro los extractos etanólicos de Schinus molle (Segura-Contreras et al., 2015), Caryophyllus aromaticus y Allium satibum (Rusin et al., 2021), así como el aceite esencial de hojas de Chenopodium ambrosioides (Kumar et al., 2017) pueden inhibir el crecimiento de L. theobromae, este último puede incluso proteger al grano de trigo del biodeterioro en la fase de poscosecha. En Sudamérica, por ejemplo, algunos cultivos de importancia agrícola donde se ha reportado el hongo son: aguacate en Perú y Cuba (Alama et al., 2006; Cabrera et al.,2016), mango en Brasil, Perú y Cuba (Marques et al., 2013; Rodríguez-Gálvez et al., 2017; Cabrera et al., 2016), cítricos en Chile, México y Cuba (Guajardo et al., 2018; Valle-de la Paz et al., 2019; Cabrera et al., 2016), papaya en Brasil (Netto et al., 2014), vid en Perú (Vergara, 2017), y teca en Brasil (Ferreira et al., 2018). Symptoms of leaf blight, stem canker, and pod rot were observed on T. cacao during a series of samplings conducted in several states of Malaysia from September 2018 to March 2019. Colonization of cashew plants by Lasiodiplodia theobromae: Microscopical features. This Dothideomycetes-related article is a stub. According to the combined ITS, tef1 and tub2 phylogeny, two isolates NI173 and NI173A from M. candolii twigs clustered with Lasiodiplodia pseudotheobromae with low support (51% ML, 52% MP) (Fig. Abdollahzadeh, J., Javadi, A., Goltapeh, E. M., Zare, R. & Phillips, A. J. L. Phylogeny and morphology of four new species of Lasiodiplodia from Iran. [ Links ], Ferreira, R. C., Marques, E., Alves, M., Martins, I., Getulio, J., & Correa, S. (2018). Y71B283261), the Yunnan Provincial Department of Human Resources and Social Security (grant no. (2013), Pavlic, T.I. 2021 Oct 11;37(11):190. doi: 10.1007/s11274-021-03137-9. (2019). De hecho, Adu-Acheampong et al. Chocolate under threat from old and new cacao diseases. Peran infeksi jamur dalam memperparah kerusakan buah kakao akibat serangan Helopeltis sp.Jurnal Perlindungan Tanaman Indonesia, 1(1), 28-32. Pakistan Journal of Biological Sciences, 4(10), 1224-1227. Lasiodiplodia magnoliae (MFLU 18-1030, holotype). This isolate did not sporulate in culture and no conidiomata were seen on the host. A Late Devonian tree lycopsid with large strobili and isotomous roots, Taxonomic and phylogenetic characterizations reveal four new species of Simplicillium (Cordycipitaceae, Hypocreales) from Guizhou, China, A molecular phylogeny of the genus Drimia (Asparagaceae: Scilloideae: Urgineeae) in India inferred from non-coding chloroplast and nuclear ribosomal DNA sequences, A new phylogeny-based tribal classification of subfamily Detarioideae, an early branching clade of florally diverse tropical arborescent legumes, Diversity of mycorrhizal Tulasnella associated with epiphytic and rupicolous orchids from the Brazilian Atlantic Forest, including four new species, Description of five new species of the Madagascan flagship plant genus Ravenala (Strelitziaceae), Insights into chloroplast genome structure, intraspecific variation, and phylogeny of Cyclamen species (Myrsinoideae), DNA barcoding and a precise morphological comparison revealed a cryptic species in the Nippolachnus piri complex (Hemiptera: Aphididae: Lachninae), The complete chloroplast genome sequences of five pinnate-leaved Primula species and phylogenetic analyses,,,,,,,,,,,,,,,,,,,,,,,,, GenBank accession number and single gene phylogenetic trees,, Metabolic analysis of the regulatory mechanism of sugars on secondary flowering in Magnolia, The primate gut mycobiome-bacteriome interface is impacted by environmental and subsistence factors, Fungal community succession on decomposing leaf litter across five phylogenetically related tree species in a subtropical forest., Ángel V. Cedeño-Moreira2  Lasiodiplodia theobromae as a causal pathogen of leaf blight, stem canker, and pod rot of Theobroma cacao in Malaysia. Forty nine strains are included in the analyses, which comprise 866 characters including gaps. Otros de los síntomas observados en campo son la pudrición y la momificación de mazorcas (Valarmathi & Ladhalakshmi, 2018). The best scoring RAxML tree with a final likelihood value of -3733.342990 is presented. K.D. β-Resorcylic acid derivatives with α-glucosidase inhibitory activity from Lasiodiplodia sp. Invasive Fungal Sinusitis by Lasiodiplodia theobromae in an Patient with Aplastic Anemia: An Extremely Rare Case Report and Literature Review. Two Lasiodiplodia pseudotheobromae strains, L. thailandica and L. endophytica clustered together and L. magnoliae formed a separate, distantly related lineage. First report of Lasiodiplodia theobromae causing leaf blight of Kadsura longipedunculata in China. They exhibit diverse life-styles as endophytes, inhabiting different asymptomatic plant tissues8,14,15, pathogens that cause diseases in various plant hosts3,16 and saprobes that are commonly found on dead woody plant tissues3,17. Hall, T. A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. 2016), L. gonubiensis (Trakunyingcharoen et al. Factors influencing quality variation in cocoa (Theobroma cacao) bean flavour profile - A review. According to Clendenin (1896), a fungus causing rot of sweet potatoes imported from Java was identified by Ellis in 1894 as a new genus and he named the fungus Lasiodiplodia tubericola. Soc. Produce Packaging for a Green Planet. Evolutionary models for phylogenetic analyses were selected independently for each locus using MrModeltest v. 3.748 under the Akaike Information Criterion (AIC). [ Links ], Statista. Plant Disease, 102(4), 818. Forests, 8(5), 145. Considering asexual characters i.e. de Silva, A.J.L. Griffon & Maul. Nucleic Acids Symposium Series 41, 95–98 (1999). 2 Universidad Técnica Estatal de Quevedo, Facultad de Ciencias Agrarias, Av. Mycelium was grown on PDA for one week at 25 °C in normal light in the laboratory. 18 de Noviembre de 2021; : En su estructura primaria no lo son. [ Links ], Vásquez-López, A., Mora-Aguilera, J., Fabricio Canchignia-Martínez2  Entre las especies de Lasiodiplodia que infectan cacao, se reportan hasta el momento L. parva (Alves et al., 2008), L. pseudo-theobromae (Serrato-Diaz et al., 2020) y L. theobromae (Asman et al., 2020), consideradas como especies crípticas según Alves et al. Other Lasiodiplodia species have been observed with only hyaline conidia such as L. chonburiensis10, L. sterculiae27 and L. thailandica in which most conidia were hyaline and only 10% were brown19. Antes de la cosecha: La aplicación de Phyton 27® genera una acción de protección curativa en frutos que padecen de infecciones latentes como mango y palto. 20, 167–186 (2005). 218p. [ Links ], Li, Y., Tsuji, S. S., Hu, M., Câmara, S. M. P., Michereff, S. J., Schnabel, G., & Chen, F. (2020). Does morphology matter in taxonomy of Lasiodiplodia? (a,b) Conidiomata on bamboo sticks in PDA culture plate. & Phillips, A. J. L. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. (2005). Experimental agriculture,48(1), 85-98. [ Links ], Martoredjo, T., Ardy, D., Hermansyah, H., & Sunardi, T. (1995). There were about 21 species. [ Links ], Muniz, C. R., Freire, F. C. O., Viana, F. M. P., Cardoso, J. E., Cooke, P., Wood, D., & Guedes, M. I. F. (2011). wrote the manuscript and A.J.L.P., J.K.L., K.D.H. The site is secure. Netto, M.W. Trakunyingcharoen, T. et al. Google Scholar. Combodia Fr. Suwannarach N, Khuna S, Kumla J, Cheewangkoon R, Suttiprapan P, Lumyong S. Plants (Basel). Canker of dogwood caused by Lasiodiplodia theobromae: a disease influenced by drought stress or cultivar selection. Griff. [ Links ], Shamsi, S., Naher, N., & Momtaz, S. (2010). Thus, de Errasti et al.30 stated that diatrypaceous endophytic fungi switch to a saprotrophic life-style during host senescence. The result is that some species are not well separated phylogenetically (Fig), such as L. hyalina and L. thailandica, L. chinensis, L. sterculiae, L. pseudotheobromae, L. pyriformis and L. crassispora. (f,g) Conidia. The aim of this study was to evaluate the incidence and prevalence of mango dieback in the region of Piura, and to identify the species of Lasiodiplodia associated with the disease . A lo largo de la historia, el cultivo del cacao (Theobroma cacao L.) se ha visto afectado por innumerables enfermedades, algunas de estas causadas por Lasiodiplodia theobromae (Pat. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Sinauer Associates, Sunderland, (2002). Es considerado uno de los cultivos más importante a nivel mundial principalmente para pequeños agricultores (Kongor, et al.,2016). H. E. Moore y Stearn] en el estado de Guerrero, México. [ Links ], Marelli, J.-P., Guest, D., Bailey, B. Hyde, sp. Fungal Divers. It might be possible to identify new distribution and host associations of Lasiodiplodia species from other forest plants in the world. These three phylogenetic studies were based on different combinations of molecular markers such as Dissanayake et al.12 who used combined ITS and tef1, Dou et al.11 used combined ITS, tef1, tub2 and rpb2 and Tibpromma et al.10 used ITS, tef1 and tub2. 2022 Aug;38(4):272-286. doi: 10.5423/PPJ.OA.01.2022.0005. ZPD-2018d Disclaimer: The NCBI taxonomy database is not an authoritative source for nomenclature or classification - please consult the relevant scientific literature for the most reliable information. 6. Phylogenetic approaches based on DNA sequence data have played a significant role in distinguishing species in Lasiodiplodia3,4,24,25. [ Links ], Shaidul, M., Most-Ferdousi, B., Montaz, A., Rafiqul, M., & Shah, M. (2001). 2013 (morphology, phylogeny, distribution, hosts); Dissanayake et al. Find diseases associated with this biological target and compounds tested against it in bioassay experiments. Barber & Mohali (2006),, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 25 November 2022, at 07:05. Lima & J.E. (2008) showed that Lasiodiplodia constitutes a clear phylogenetic lineage. Rosa, aguacate, papaya, Phoenix spp. Este tipo de cookies son las que, por ejemplo, nos permiten identificarte, darte acceso a determinadas partes restringidas de la página si fuese necesario, o recordar diferentes opciones o servicios ya seleccionados por ti, como tus preferencias de privacidad. Phillips acknowledges the support from UID/MULTI/04046/2019 Research Unit grant from FCT, Portugal to BioISI. China) following the manufacturer’s protocol. Toxins, 12(7), 457. Mycologia, 98(3), 423-435. Dissertatação de Mestrado, Instituto Agronômico de Campinas. Genetic Characterization of the Cacao Cultivar CCN 51: Its Impact and Significance on Global Cacao Improvement and Production. Sin embargo, L. theobromae parece ser la especie más importante, estudiada y citada. Existe evidencias de la existencia de resistencia en cacao a la muerte regresiva causada por L. theobromae. Lasiodiplodia theobromae causes vascular streak dieback (VSD)-like symptoms of cacao in Davao Region, Philippines. [ Links ], Ghini, R. (1993). Fitopatologia Brasileira, 31(6), 572-578 En J. Martínez Herrera., M.A., Ramírez Guillermo., J. Cámara-Córdova (Eds). Citrus sp. Conidiomata not observed on bamboo sticks on PDA, MEA or Water Agar. Caracol africano en Venezuela: ¿Qué hacer para exterminarlo. Lima 18 - Perú. (2016) listed 31 species known from culture. (2021). volume 9, Article number: 14355 (2019) Saprobic Lasiodiplodia species have been recorded such as Lasiodiplodia iraniensis on dead twigs of Salvadora persica, L. hormozganensis on Olea sp.3 and L. theobromae on dead twigs of Eucalyptus sp.17. 2014CB954101). IOP Conference Series: Earth and Environmental Science, 486, 012158. A-B, Crecimiento del patógeno en placas de Petri conteniendo medio de cultivo Papa Dextrosa Agar (PDA), mostrando un aspecto algodonoso con rápida extensión micelial (superficie completamente cubierta). Journal of Phytopathology, 106(3), 204-213. Agrociencia, 43, 717-728. [ Links ], Ogundana, S. K. (1983). H. E. Moore and Stearn] grafts in México. Endophytes are a hidden bioresource of fungal diversity that have the potential to produce important bioactive agents15. El movimiento de material vegetal infectado por Botryosphaeriaceae incluido L. theobromae mediado por humanos, puede facilitar su dispersión a nivel mundial (Mehl et al., 2017). Agronomy, 43, e44785. Especificaciones Técnicas / Modo de acción. Wan-Hao Chen, Yan-Feng Han, … Zong-Qi Liang, Manuel de la Estrella, Félix Forest, … Anne Bruneau, Emiliane Fernanda Silva Freitas, Meiriele da Silva, … Maria Catarina Megumi Kasuya, Thomas Haevermans, Annette Hladik, … Patrick Blanc, Mariusz Kanturski, Yerim Lee, … Seunghwan Lee, Scientific Reports (2014). Cramer, Berlin. The pathogenic fungus can cause young twigs death (a characteristic symptom found in field conditions), fruits rot and mummification, and even vascular dieback. Phillips & K.D. Lasiodiplodia theobromae: an emerging threat to cocoa causes dieback and canker disease in Sulawesi. Phyton 27® ayuda a prevenir el ingreso de hongos. (d) Peridium. Cite this article. Persian lime (Citrus latifolia Tan.) performed the morphological study and phylogenetic data analyses with help from A.J.L.P. (2020). government site. 1 Facultad de Ingeniería Agronómica, Universidad Técnica de Manabí, Campus Experimental La Teodomira, km 13, Lodana, Santa Ana. Condiciones agroclimáticas óptimas para el patógeno. (a) Conidiomata on bamboo sticks in PDA culture plate. ficha de generos: genero: lasiodiplodia . Faces of Fungi numbers and Index Fungorum numbers were registered as described in Jayasiri et al.38 and Index Fungorum (2019)39. [ Links ], Martínez de la Parte, E., & Pérez-Vicente, L. (2015). Pathogenicity on Persian lime young plants using a mycelial plug inoculation method showed that all identified Lasiodiplodia species were able to cause necrotic lesions and gummosis, but L. subglobosa, L. iraniensis, and L. pseudotheobromae were the most virulent. Cladosporium está ocasionando daños en palto y mango en los valles de Áncash, Uva de mesa: crece la necesidad de usar biofungicidas en el control del oídio, El oídio del arándano afecta a cuatro zonas productoras del país, SGS proyecta que crecerá en 50% su facturación en análisis de pesticidas. Cuando los niveles de daño son muy grandes, no hay un posible control, por más que se use fungicida que llegue a la zona necrosada. Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. (2019). (1849) 2006). Para describir cada uno de los métodos de manejo de la muerte regresiva y de la pudrición de mazorcas causadas L. theobromae en cacao, se optó por usar artículos donde hayan abordado su manejo, independientemente del cultivo abordado. Sequence analyses were carried out using the Maximum Likelihood and Bayesian Inference methods. Chen et al.15 chemically investigated a strain of Lasiodiplodia sp. 2013). Lasiodiplodia, commonly referred to as black-soot disease, is a significant pathogen in tropical forestry.[2]. Interestingly, we isolated one endophytic and 2 saprobic isolates of Lasiodiplodia pseudotheobromae. Studies in Mycology, 76, 31-49. 2015) and L. lignicola (Phillips et al. According to Clendenin (1896), a fungus causing rot of sweet potatoes imported from Java was identified by Ellis in 1894 as a new genus and he named the fungus Lasiodiplodia tubericola. Conidiophores absent. Bioinformatics 14, 817–818, (1998). [ Links ], Kumar, R., Mishra, A., Dubey, N., & Tripathi, Y. (2019), donde se investigaron 13061 genes codificadores de proteínas en 52 cepas de L. theobromae aislados de tejidos sintomáticos de cacao, se encontraron 2862 exclusivos para la especie en estu dio, en comparación con otras miembros de Botryos-phaeriaceae estrechamente relacionadas. Lasiodiplodia magnoliae has longer conidia (24–30 μm) than L. mahajangana (14–24 μm)18. Dr. Shaun Pennycook is thanked for checking species name. Australas. Phillips, A. J., Hyde, K. D., Alves, A. and transmitted securely. A., Evans, H. C., Brown, J. K., et al. De Silva, N. I. et al. E-F, Mazorcas infectadas en condiciones de campo, mostrando pudrición parcial (E) y total (F). Câmara (2014), F.J.J. Australasian Plant Disease Notes, 12(1), 54. Curtis) Abbas, B. Sutton, Ghaffar & Abbas (2004), T.I. & Maubl) problem and their control strategy in Indonesia. An official website of the United States government. Yang, T. et al. Alves, A., Crous, P. W., Correia, A. Characterization of difenoconazole resistance in Lasiodiplodia theobromae from papaya in Brazil. Studies in Mycology 76, 51–167, (2013). cantalupensis Naudin) e avocado (Persea americana) commercializati in Italia.) Red Globe, en Sullana-Piura. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. D, Hifas septadas de color marrón oscuro. Marais (2014), (Berk. Article  71, 201–214, (2015). Maximum parsimony analysis of the remaining 133 parsimony informative characters resulted in 1000 equally parsimonious trees of 535 steps with CI = 0.632, RI = 0.798, RC = 0.504 and HI = 0.368., DOI: Keywords: Theobroma cacao L.; Lasiodiplodia theobromae; biology cycle; vascular dieback; pod rot; management measures. ZPD-2018c Lasiodiplodia sp. & Menezes, M. (2005). Palabras clave: Theobroma cacao L.; Lasiodiplodia theobromae; ciclo biológico; muerte regresiva; pudrición de mazorcas; medidas de manejo. L. theobromae es un hongo patógeno que ha tomado importancia en los diferentes países de América Latina, especialmente Ecuador, causando principalmente muerte regresiva y pudrición de frutos en cultivos comerciales de cacao. Fungal Diversity, 67(1), 127-141. White, T., Bruns, T., Lee, S. & Taylor, J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. & Menezes, M. Tibpromma, S. et al. Marques, A.J.L. [ Links ], Pitt, W. M., Huang, R., Steel, C. C., & Savocchia, S. (2013). The recent multi locus phylogenetic approaches with ITS, tef1 and tub2 nucleotide sequence data has advanced the recognition of numerous Lasiodiplodia species with high phylogenetic support3,4,10,11. 15 días antes de la poda: para producir un descenso en el potencial de inóculo presente en el campo y además proteger preventivamente a la planta. Las cookies técnicas son estrictamente necesarias para que nuestra página web funcione y puedas navegar por la misma. [ Links ], Li, Z., Wang, Y.-T., Gao, L., Wang, F., Ye, J.-L., & Li, G.-H. (2014). Manejo Integrado de Doenças Radiculares. Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants. *  Figura 2 Síntomas causados por Lasiodiplodia theobromae en cacao. Wir bitten um Entschuldigung für die Unannehmlichkeit. Mien tras tanto, a nivel bioquímico se conoce que L. theobromae posee 134 compuestos químicamente defini dos pertenecientes a las clases de metabolitos secunda rios y ácidos grasos, i.e. Parameters for maximum likelihood were set to rapid bootstrapping and the analysis carried out using 1000 replicates. One species (Lasiodiplodia magnoliae) was considered to be saprobic, while the other (L. endophytica) was thought to be endophytic. ISSN 2045-2322 (online). [ Links ], Twumasi, P., Ohene-Mensah, G., & Moses, E. (2014). Esta práctica en conjunto con otras detalladas en esta revisión puede resultar muy efectiva para el control de L. theobromae en el campo, ya que al ser un patógeno de comportamiento monocíclico, se debe actuar al inicio de su ciclo de vida (Uc-Várquez et al., 2017). Three new Lasiodiplodia spp. Por ejemplo, recientemente Mvondo et al. This site needs JavaScript to work properly. GENERALIDADES PROCURE 480 SC es un fungicida de acción sistémica localizada y traslaminar, autorizado para uso agrícola en diversos cultivos y en forma muy preponderante para el control de cenicilla. Plant Dis. Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. Disease symptoms – Diebacks, cankers, fruit rots. Aunque no existe ningún trabajo mostrando el uso de este método en el control de enfermedades causadas por L. theobromae, la solarización del suelo podría ser una interesante alternativa en la etapa de propagación de plantas de cacao, debido a la reducción del inóculo localizado en el sustrato (Katan et al., 1976; Michereff et al., 2005). The internal transcribed spacers (ITS), part of the translation elongation factor-1α (tef1) and partial β-tubulin (tub2) sequence data were analyzed to investigate the phylogenetic relationships of the new species with other Lasiodiplodia species. Striodiplodia Zambett. Otros de los métodos culturales que pueden ser implementados, están la selección del área de plantación libre de patógenos, la elección de la época de siembra (vivero) o trasplante (campo), el uso de material de propagación libre de patógenos, y tal vez uno de los más importantes, evitar lesiones en las plantas, puesto que las heridas pueden ser la principal puerta de ingreso para el patógeno (Michereff et al., 2005). [ Links ], Evans, H. C., Holmes, K. A., Thomas, S. E. (2003). Resumen. Lasiodiplodia pandanicola was isolated from dead leaves of Pandanus in Thailand10. (2017). 2013) with clear evidence that connects sexual with asexual morphs. Thai Forest Bulletin (Botany) 37, 111–138 (2009). [ Links ], Zhang, W., Yan, J., Li, X., Xing, Q., Chethana, K. W. T., & Zhao, W. (2019). Duri … Pesticide Biochemistry and Physiology, 162, 60-68. Sin embargo, si la planta atraviesa algún tipo de estrés los síntomas se pueden hacer visibles (Mullen, 1991). Autos nuevos, Autos clásicos, Autos de carrera, fotos de autos, test drives, ficha técnica, comparativas, videos y curiosidades. A lo largo de la historia, el cultivo del cacao (Theobroma cacao L.) se ha visto afectado por innumerables enfermedades, algunas de estas causadas por Lasiodiplodia theobromae (Pat.) European Journal of Plant Pathology, 132(4), 489-498. & Kohn, L. M. A method for designing primer sets for speciation studies in filamentous ascomycetes. Las mazorcas afectadas presentan en su interior una masa de micelio negruzco que envuelve las almendras (Figura 2H). Lasiodiplodia. Pakistan Journal of Botany, 37, 1023-1030. 120, 225–234 (1992). The study has expanded the knowledge of Lasiodiplodia species providing two novel species and two new host records. Persoonia 34, 87–99, (2015). En vista de la poca información científica acerca de L. theobromae en cacao a nivel mundial, se torna importante elucidar varias interrogantes sobre su etiología y manejo, especialmente en países sudamericanos. American Phytopathological Society Press. Fungal Biology 121, 322–346 (2016). Flora-Morphology, Distribution, Functional Ecology of Plants 209, 704–710, (2014). 15 días después de la poda: Su aplicación contribuye a frenar el avance de cualquier hongo fitopatógeno que haya ingresado durante la poda. 2016 Dec;181(11-12):901-908. doi: 10.1007/s11046-016-0062-z. Conidial dimensions of the endophytic isolate (26–31 × 10–12 μm) overlap with those of the ex-type isolate. (2013). The tree is rooted with Diplodia mutila (CMW 7060). In 2015, symptomatic samples were collected from 12 commercial Persian lime orchards, and 60 Lasiodiplodia isolates were obtained. Fungal Divers. Phyton 27 ® es el único sulfato de cobre pentahidratado de acción sistémica en el mercado. (2015). Swofford, D. L. PAUP: phylogenetic analysis using parsimony, version 4.0 b10. (pp. [ Links ], Slippers, B., Boissin, E., Phillips, A., Groenewald, J., Lombard, L., et al. (2021). Clendenin (1896) provided a description of the genus and the species, attributing both to Ellis and Everhardt. Fig. Please enable it to take advantage of the complete set of features! Chemical control of Lasiodiplodia theobromae, the causal agent of mango decline in Sindh. Pathogenicity and in vitro control of Lasiodiplodia theobromae and Fusarium sp., pathogens associated with cocoa dieback in Cameroon. Thank you for visiting (2004) could not locate the types, and they could not find any specimens from the original hosts or origins. Lasiodiplodia mahajangana was isolated from Terminalia catappa in Madagascar18. Este método consiste en el uso de factores físicos (por lo general temperatura y radiación) para controlar enfermedades. Difenoconazol. (c) Squash mount of conidiomata (d) Conideogeous cell (e) Paraphyses (f–j) Conidia. Finalmente, Sudha et al. African Journal of Microbiology Research, 8(33), 3094-3100. Pinterest. AMISTAR® TOP combina la destacada acción preventiva y antiesporulante de la azoxistrobina, perteneciente al grupo de las estrobirulinas, con el efecto erradicante de difenoconazole, perteneciente al grupo de los triazoles. (k) Brown conidia on the surface of host. Phillips, A. Alves & Abdollahz. Lasiodiplodia Ellis & Everh. Phylograms were visualized with FigTree v1.4.0 Rambaut49 and annotated in Microsoft Power Point (2010). (e) Conidiogenous cells and paraphyses. Biological Control, 15(3), 235-240. Lasiodiplodia magnoliae is phylogenetically sister to L. mahajangana and L. pandanicola but morphologically distinct from L. mahajangana in having larger conidia. (1955), Lasiodiplodia is a genus of fungi in the family Botryosphaeriaceae. Classification – Dothideomycetes, incertae sedis, Botryosphaeriales, Botryosphaeriaceae, Type species – Lasiodiplodia theobromae (Pat.) Fungal Biol Rev. In cocoa, L. theobromae can survive in soil and culture remains in the form of pycnidia and chlamydospores, spreading by different factors until reaching the plant tissues, remaining there as an endophyte. 2013). HHS Vulnerability Disclosure, Help C, Planta evidenciando muerte regresiva. There are 55 epithets of Lasiodiplodia recorded in Index Fungorum (March 2019) and 43 species names in MycoBank (March 2019). Griffon & Maul. [ Links ], Rodrigues, R. (2003). Two new species of Lasiodiplodia (Lasiodiplodia endophytica and Lasiodiplodia magnoliae) are described and illustrated from Magnolia forests in Yunnan, China. Fr. Plant Disease, 75(9), 886-889. Las esporas contenidas en los picnidios son liberadas al ambiente en condiciones óptimas (Muhamad et al., 2009), siendo diseminadas por el viento, la lluvia (Vásquez-López et al., 2009) o transportadas a través de herramientas e insectos de la familia Miridae (Helopeltis thetvora, H. ammonli y H. theobromae) (Kranz et al., 1977; Ploetz, 2003, Figura 3). Chen, S. et al. Available at, (accessed 1 January 2019) (2012). Comparison of total length of 477 bases of ITS sequences revealed one base pair difference among three strains and one base deletion in L. endophytica. Genomic DNA was extracted from the mycelium using a Biospin fungus genomic DNA kit (BioFlux®, P.R. Elevation ranges from 709–869 m and mean temperature and precipitation are 21.0 °C and 1532 mm respectively. Micron, 42(5), 419-428. [ Links ], Marques, M. W., Lima, N. B., de Morais, M. A., Barbosa, M. A. G., Souza, B. O., et al. Esta es una gran alternativa al momento de prevenir alguna infección fúngica, pudiendo en algunos casos ser comparado con el control químico (Mortuza & Ilag, 1999). Maximum parsimony was run with the heuristic search option, random taxon addition, tree bisection-reconnection (TBR) for the branch swapping algorithm and 1000 random sequence additions, with maxtrees set at 1000. Frontiers in Microbiology, 9, 829. Organisms Diversity and Evolution 12, 335–337, (2012). (2008), M.S.B. Ecuador. Tabla 1 Estado de la resistencia a fungicidas en aislados de Lasiodiplodia theobromae en diferentes cultivosÂ, g. Manejo integrado de enfermedades (MIE). de Silva, N.I., Phillips, A.J.L., Liu, JK. (2020). Botryodiplodia theobromae Pat] in the cocoa crop: symptoms, biological cycle, and strategies management, Anthony A. Moreira-Morrillo1  Federal government websites often end in .gov or .mil. 1). Use the Previous and Next buttons to navigate three slides at a time, or the slide dot buttons at the end to jump three slides at a time. 2021 Oct 8;10(10):1297. doi: 10.3390/pathogens10101297. Arx, J. Este método consiste básicamente en interferir los procesos del patógeno como sobrevivencia, diseminación y reproducción, minimizando los efectos de la enfermedad. Algunas enfermedades re portadas en cultivos de importancia agrícola son pudri ción de raíz (Sathya et al., 2017), gomosis (Guajardo et al., 2018), cáncer, muerte descendente (Asman et al., 2020), tizón de la hoja (Fan et al., 2020) y pudrición de mazorcas (Puig et al., 2021). 21, 90–106, (2007). Commonwealth Mycological Institute, Kew, Surrey, England. Revista Árvore, 42(3), e420304. phylogeographic analysis of the globally distributed tree pathogen Lasiodiplodia theobromae. Quality of PCR products was checked on 1% agarose electrophoresis gels stained with ethidium bromide. The sexual morph has been reported for L. theobromae, but the connection with the asexual morph has not been confirmed (Phillips 2013). [ Links ], Kranz, J., Schmutterer, H., & Koch, W. (1978). En Bailey B. Phytophthora palmivora Causing Disease on Theobroma cacao in Hawaii. Lasiodiplodia microconidia Taxonomy ID: 2930956 (for references in articles please use NCBI:txid2930956) current name. Lasiodiplodia theobromae causes a damaging dieback of cocoa in India. (2021). Ex-type (ex-epitype) strains are in bold and marked with an asterisk* and voucher strains are in bold. Neither Patouillard (1892) nor Clendenin (1896) referred to any type or other specimens of the genus or species. [ Links ], Soria, V. J. provided funding for the study. Estimated base frequencies were as follows; A = 0.211797, C = 0.285190, G = 0.260783, T = 0.242230; substitution rates AC = 0.983905, AG = 3.303939, AT = 1.281593, CG = 0.950258, CT = 5.553417, GT = 1.000000; gamma distribution shape parameter α = 0.221126. Cacao usage by the earliest Maya civilization. In this study two new species of Lasiodiplodia were identified and described from Magnolia candolii in the southern part of Yunnan Province, China. Netto, M.W. Jurnal Perlindungan Tanaman Indonesia, 23(1), 133-141. Lasiodiplodia magnoliae MFLUCC 18-0948 formed a separate clade with L. citricola IRAN1522C. Slippers, B. Species of Lasiodiplodia associated with mango in Brazil. Conidiogenous cells 2–5 µm diam., hyaline, discrete, smooth and cylindrical. B. Marques & A.J.L. St. Paul, Minnesota, USA. En el duraznero, se ha reportado que la alta humedad y heridas mecánicas promueven el desarrollo de la gomosis ocasionada por L. theobromae (Li et al., 2014).  Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons, Av. DNA sequence comparisons of ITS and tef1 among L. endophytica, L. iraniensis and L. thailandica are given in Table 2. In Embrapa Semiárido-Resumo em anais de congresso (ALICE). Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand, Nimali I. de Silva, Saisamorn Lumyong & Kevin D. Hyde, Biodiversity and Ethnobiology, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand, Center of Excellence in Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand, Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650201, P.R. Thus, L. magnoliae and L. chonburiensis are closely related and found in one clade. Article  International Journal of Current Research in Biosciences and Plant Biology, 6(7), 1-13. The amplified PCR fragments were sequenced by Sangon Biotech (Shanghai) Co., Ltd, P.R. We did not observe any pathogenic Lasiodiplodia species in our study. Cuarenta y ocho especies de la flora de Chiapas incluidas . Griffon & Maubl., agente causal das podridões de tronco e raízes da videira. The genetic diversity of cacao and its utilization. Alves et al. Lasiodiplodia theobromae (Pat. Fungi were isolated from dead twigs attached to the host. Previous studies have recorded both hyaline and pigmented conidia in L. pseudotheobromae6 and L. thailandica21. A., Guadalupe, I., Bayman, P., & Goenaga, R. (2020). (2019) demostró el efecto inhibidor del extracto etanólico (100 mg L-1) de semillas de neem (Azadirachta indica), consiguiendo reducir el crecimiento de L. theobromae en plantas de cacao, relacionado probablemente al contenido de Azadiractina en el extracto. Dissanayake, A. J. et al. Griffin and Maublanc (1909) considered that on account of the pycnidial paraphyses, Botryodiplodia theobromae, described by Patouillard (1892), was more suitably accommodated in Lasiodiplodia. [ Links ], Okey, E. N., Akwaji, P. I., Umana, E. J., & Omini, J. U. Taxonomy information for Lasiodiplodia. Endophytes and mycoparasites associated with an indigenous forest tree. (a,b) Conidiomata on bamboo sticks in PDA culture plate. (2019). Phillips, A. J. L. et al. (2013) differentiated 18 species in Lasiodiplodia on the basis of conidial morphology (especially dimensions) and morphology of the paraphyses, in reality, species in Lasiodiplodia cannot be identified with any confidence from their morphology and molecular data are necessary for definitive identifications. References: Phillips et al. Persoonia. picture_as_pdf Ficha técnica Amistar Top (92.29 KB) download. 489, P-7, Col. Hipódromo, Alc. designed the study. De manera general, el manejo de patógenos de la familia Botryosphaeriaceae es complejo, más aún cuando el patógeno ya está presente en el local de cultivo. Epub 2019 Oct 28. It was considered here that phylogeny based on combined ITS, tef1 and tub2 sequence data provides sufficient evidence for the designation of L. endophytica (S8) as a novel taxon.
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